The star who is best known in Britain for his time with Arsenal, where he was part of the club's 'Invincibles' squad which won the Premier League undefeated in 2004, was currently playing for Extremadura in the Spanish second division.
He had also played for the likes of Real Madrid, Atletico Madrid, Sevilla, Espanyol and Benfica during his illustrious career, where he won numerous titles and earned 21 caps for the Spanish national side.
According to a tweet posted by Sevilla, Reyes died after being involved in a traffic collision on Saturday morning.
Arsenal have also paid tribute to the star, with many former players and colleagues expressing their sympathies over his passing.
Elsewhere, fans of numerous clubs have paid tribute to Reyes, with some even sharing important goals and memories from his time spent in London and Madrid.