The Netflix show did halt production of the show "as a mark of respect" for the late monarch, and it was not up for awards this year.
However, some didn't seem to mind that the monarch was left out and made jokes, including Succession creator and writer Jesse Armstrong who took a swipe at King Charles III.
The British screenwriter for the HBO television series was giving his acceptance speech for the show, which was awarded Best Drama Series.
Within the speech, he joked that it was a big week for "successions," referring to King Charles automatically assuming the throne following the death of his mother.
And despite the nation being in a state of national mourning following the death of its longest-reigning monarch, Armstrong said that there was "a bit more voting involved" in Succession winning their Emmy than King Charles' getting on the throne.
"Big week for Succession. New king in the UK this week for us. Evidently, a little more voting involved in our winning than Prince Charles," he said.
This joke provoked a ripple of reaction in the theatre, including murmurs of discontent echoing around the room.
Some in the crowd awkwardly laughed while one person was heard cheering.
Scottish actor Brian Cox quickly chimed in with: "Keep it royalist, keep it royalist!'"
\u201canti monarchist king jesse armstrong \u201d
— clara \ud83e\udd0d (@clara \ud83e\udd0d)
The sentiments also trickled onto social media, with many pointing out the inappropriate timing of his joke.
Others blasting his' sleazy, snotty and obscene' quips.
Armstrong, who is also known for writing British comedies The Thick of It and Peep Sho', continued on to say: 'I'm not saying that we're more legitimate in our position than he is; we'll leave that to other people. We are incredibly grateful to have this; it's a wonderful honor."
The royal family announced the passing of Queen Elizabeth, aged 96, on 8 September at Balmoral Castle.
\u201cThe Queen died peacefully at Balmoral this afternoon.\n\nThe King and The Queen Consort will remain at Balmoral this evening and will return to London tomorrow.\u201d
"We mourn profoundly the passing of a cherished sovereign and a much-loved mother. I know her loss will be deeply felt throughout the country, the Realms and the Commonwealth, and by countless people around the world," he said in a statement.
Have your say in our news democracy. Click the upvote icon at the top of the page to help raise this article through the indy100 rankings.